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david sabatini  (Addgene inc)


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    Structured Review

    Addgene inc david sabatini
    David Sabatini, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/david+sabatini/Human+CRISPR+Metabolic+Gene+Knockout+Library+(Pooled+Library+%23110066)/pmc12998000-154-11-13
    Average 93 stars, based on 27 article reviews
    david sabatini - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    CRISPR:

    Article Title: Targeting HIF-2α in colorectal cancer reveals a cholesterol biosynthesis-dependent ferroptotic vulnerability.
    Article Snippet: .. The human CRISPR metabolic gene knockout library was a gift from David Sabatini (Addgene, 110066) [20]. ..

    Article Title: Targeting HIF-2α in colorectal cancer reveals a cholesterol biosynthesis–dependent ferroptotic vulnerability
    Article Snippet: .. The human CRISPR metabolic gene knockout library was a gift from David Sabatini (Addgene, 110066) [ ]. ..

    Article Title: Mitochondrial Integrated Stress Response Activation Creates a Therapeutic Vulnerability to MCL-1 Inhibition in Acute Myeloid Leukemia
    Article Snippet: .. The Human CRISPR Metabolic Gene KO Library was a gift from David Sabatini (Addgene #110066) (ref. ). ..

    Gene Knockout:

    Article Title: Targeting HIF-2α in colorectal cancer reveals a cholesterol biosynthesis-dependent ferroptotic vulnerability.
    Article Snippet: .. The human CRISPR metabolic gene knockout library was a gift from David Sabatini (Addgene, 110066) [20]. ..

    Article Title: Targeting HIF-2α in colorectal cancer reveals a cholesterol biosynthesis–dependent ferroptotic vulnerability
    Article Snippet: .. The human CRISPR metabolic gene knockout library was a gift from David Sabatini (Addgene, 110066) [ ]. ..

    Plasmid Preparation:

    Article Title: Regulation of tension-dependent localization of LATS1 and LATS2 to adherens junctions
    Article Snippet: The psPAX2 and pMD2.G plasmids for lentiviral production were a gift from Didier Trono (Addgene plasmid # 12260 and Addgene plasmid # 12259). .. Lentiviral vector pLJM1-EGFP containing a puromycin resistance gene was used to generate stable cell lines expressing various GFP-fusion proteins. pLJM1-EGFP was a gift from David Sabatini (Addgene plasmid # 19319) [ ]. .. Vectors pIC113 and pcDNA3.1(+) (Invitrogen) were used to produce constructs used for transient expression in HEK293 derived cell lines.

    Article Title: Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3
    Article Snippet: .. The mutations were designed based on previously published studies that showed loss of IFN-β induction [ – ]. pLJM1-tdTomato construct was generated from pLJM1-GFP, a gift from David Sabatini (Addgene plasmid number 19319; http://n2t.net/addgene:19319 ; RRID, Addgene_19319) [ ]. .. All constructs were generated by Gibson cloning with HiFi assembly reagent (New England Biolabs, E2621)

    Article Title: Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3.
    Article Snippet: .. The mutations were designed based on previously published studies that showed loss of IFN-β induction [79–81]. pLJM1-tdTomato construct was generated from pLJM1-GFP, a gift from David Sabatini (Addgene plasmid number 19319; http:// n2t.net/addgene:19319; RRID, Addgene_19319) [82]. .. All constructs were generated by Gibson cloning with HiFi assembly reagent (New England Biolabs, E2621) Cell lines, reagents, and treatments All cells were cultured at 37°C and 5% CO 2 .

    Stable Transfection:

    Article Title: Regulation of tension-dependent localization of LATS1 and LATS2 to adherens junctions
    Article Snippet: The psPAX2 and pMD2.G plasmids for lentiviral production were a gift from Didier Trono (Addgene plasmid # 12260 and Addgene plasmid # 12259). .. Lentiviral vector pLJM1-EGFP containing a puromycin resistance gene was used to generate stable cell lines expressing various GFP-fusion proteins. pLJM1-EGFP was a gift from David Sabatini (Addgene plasmid # 19319) [ ]. .. Vectors pIC113 and pcDNA3.1(+) (Invitrogen) were used to produce constructs used for transient expression in HEK293 derived cell lines.

    Expressing:

    Article Title: Regulation of tension-dependent localization of LATS1 and LATS2 to adherens junctions
    Article Snippet: The psPAX2 and pMD2.G plasmids for lentiviral production were a gift from Didier Trono (Addgene plasmid # 12260 and Addgene plasmid # 12259). .. Lentiviral vector pLJM1-EGFP containing a puromycin resistance gene was used to generate stable cell lines expressing various GFP-fusion proteins. pLJM1-EGFP was a gift from David Sabatini (Addgene plasmid # 19319) [ ]. .. Vectors pIC113 and pcDNA3.1(+) (Invitrogen) were used to produce constructs used for transient expression in HEK293 derived cell lines.

    Construct:

    Article Title: Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3
    Article Snippet: .. The mutations were designed based on previously published studies that showed loss of IFN-β induction [ – ]. pLJM1-tdTomato construct was generated from pLJM1-GFP, a gift from David Sabatini (Addgene plasmid number 19319; http://n2t.net/addgene:19319 ; RRID, Addgene_19319) [ ]. .. All constructs were generated by Gibson cloning with HiFi assembly reagent (New England Biolabs, E2621)

    Article Title: Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3.
    Article Snippet: .. The mutations were designed based on previously published studies that showed loss of IFN-β induction [79–81]. pLJM1-tdTomato construct was generated from pLJM1-GFP, a gift from David Sabatini (Addgene plasmid number 19319; http:// n2t.net/addgene:19319; RRID, Addgene_19319) [82]. .. All constructs were generated by Gibson cloning with HiFi assembly reagent (New England Biolabs, E2621) Cell lines, reagents, and treatments All cells were cultured at 37°C and 5% CO 2 .

    Generated:

    Article Title: Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3
    Article Snippet: .. The mutations were designed based on previously published studies that showed loss of IFN-β induction [ – ]. pLJM1-tdTomato construct was generated from pLJM1-GFP, a gift from David Sabatini (Addgene plasmid number 19319; http://n2t.net/addgene:19319 ; RRID, Addgene_19319) [ ]. .. All constructs were generated by Gibson cloning with HiFi assembly reagent (New England Biolabs, E2621)

    Article Title: Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3.
    Article Snippet: .. The mutations were designed based on previously published studies that showed loss of IFN-β induction [79–81]. pLJM1-tdTomato construct was generated from pLJM1-GFP, a gift from David Sabatini (Addgene plasmid number 19319; http:// n2t.net/addgene:19319; RRID, Addgene_19319) [82]. .. All constructs were generated by Gibson cloning with HiFi assembly reagent (New England Biolabs, E2621) Cell lines, reagents, and treatments All cells were cultured at 37°C and 5% CO 2 .



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    93
    Addgene inc david sabatini
    David Sabatini, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/david+sabatini/Human+CRISPR+Metabolic+Gene+Knockout+Library+(Pooled+Library+%23110066)/pmc12998000-154-11-13
    Average 93 stars, based on 1 article reviews
    david sabatini - by Bioz Stars, 2026-09
    93/100 stars
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